Publications

Selected Publications

Tissue-engineered transcatheter vein valve
Syedain, Z.H., Jenson, A., Patel, P., Feagler, C., Bahmer, L., Faizer, R. and R. T. Tranquillo, Biomaterials, 216: 119229 (2019)

Shear conditioning of adipose stem cells for reduced platelet binding to engineered vascular grafts
La, A. and R. T. Tranquillo, Tissue Eng Part A, 2018. 24(15-16): p. 1242-1250.

A cardiac patch from aligned microvessel and cardiomyocyte patches
Schaefer, J.A., Guzman, P.A., Riemenschneider, S.B., Kamp, T.J. and R.T. Tranquillo,  J Tissue Eng Regen Med 12:546-566 (2018).

A completely biological ‘off-the-shelf’ arteriovenous graft that recellularizes in baboons
Syedain, Z.H., Graham, M.L., Dunn, T.B., O’Brien, T., Johnson, S.L., Schumacher, R.J. and R. T. Tranquillo, Science Transl Med 9(414): 10.1126/scitranslmed.aan4209 (2017).

Implantation of a tissue-engineered tubular heart valve in growing lambs
Reimer, J.M.; Syedain, Z.H.; Haynei, B.; Lahti, M.; Berry, J.; and Tranquillo, R.T.;
Ann Biomed Eng, 45(2):439-451 (2017) doi: 10.1007/s10439-016-1605-7.

Tissue engineering of acellular vascular grafts capable of somatic growth in young lambs
Syedain, Z.H.; Reimer, J. M.; Lahti, M.; Berry, J.; Johnson, S.; and Tranquillo, R.T.; 
Nat Commun 7:12951 (2016), doi:10.1038/ncomms12951.

Inosculation and perfusion of pre-vascularized tissue patches containing aligned human microvessels after myocardial infarction
Riemenschneider, S.B.; Mattia, D.J.; Wendel, J.S.; Schaefer, J.A.; Ye, L.; Guzman, P.A.; and Tranquillo, R.T. ; Biomaterials 97:51-61 (2016), doi: 10.1016/j.biomaterials.2016.04.031

Cyclic stretch and perfusion bioreactor for conditioning large diameter engineered tissue tubes
Schmidt, J.B. and Tranquillo, R.T.
Ann Biomed Eng 44(5):1785-97 (2016), doi: 10.1007/s10439-015-1437-x.

Tissue contraction force microscopy for optimization of engineered cardiac tissue
Schaefer, J.A. and R.T. Tranquillo
Tissue Eng Part C 22:76-83 (2016), doi: 10.1089/ten.TEC.2015.0220.

6-month aortic valve implantation of an off-the-shelf tissue-engineered valve in sheep
Syedain, Z.H.; Reimer, J.M.; Schmidt, J.B.; Lahti, M., Berry, J.; Bianco, R.; and Tranquillo, R.T.
Biomaterials 73:175-84 (2015), doi: 10.1016/j.biomaterials.

Functional effects of a tissue-engineered cardiac patch from human induced pluripotent stem cell-derived cardiomyocytes in a rat infarct model
Wendel, J.; Ye, L.; Rao, T.; Zhang, J.; Zhang, J.; Kamp, T.J.; and Tranquillo, R.T.
Stem Cells Transl Med 4(11):1324-32 (2015), doi: 10.5966/sctm.2015-0044.

Effects of intermittent and incremental cyclic stretch on ERK signaling and collagen production in engineered tissue
Schmidt, J.B.; Chen, K.; and Tranquillo, R.T.
Cell Molec Bioeng 9:55-64 (2015), doi: 10.1007/s12195-015-0415-6.

Pediatric tubular pulmonary heart valve from decellularized engineered tissue tubes
Reimer, J.M.; Syedain, Z.H.; Haynie, B; and Tranquillo,R.T.
Biomaterials 62: 88-94 (2015), doi: 10.1016/j.biomaterials.2015.05.009. 

Automated image analysis programs for the quantification of microvascular network characteristics
Morin, K.T.; Carlson, P. C.; and Tranquillo, R. T.
Methods 84: 76-83 (2015), doi: 10.1016/j.ymeth.2015.03.014.

A mathematical model for understanding fluid flow through engineered tissues containing microvessels
Morin, K.T.; Lenz, M.S.; Labat, C.; and Tranquillo, R.T. 
J Biomech Eng 137(5): 051003 (2015), doi: 10.1115/1.4029236.

Influence of culture conditions and extracellular matrix alignment on human mesenchymal stem cell invasion into decellularized engineered tissues
Weidenhamer, N.K.; Moore, D.L.; Lobo, F.L.; Klair, N.T.; and Tranquillo, R.T.
J Tissue Eng Regen Med 9(5): 605-18 (2015), doi: 10.1002/term.1974.

Engineered microvessels possessing alignment and high lumen density via cell-induced fibrin gel compaction and interstitial flow
Morin, K.T.;Dries-Devlin, J.L.; and Tranquillo; R.T. 
Tissue Eng Part A 20: 553-65 (2014), doi: 10.1089/ten.TEA.2013.0262.

Functional consequences of a tissue-engineered myocardial patch for cardiac repair in an acute rat infarct model
Wendel, J.; Ye, L.; Zhang, P.; Tranquillo, R.T.; and Zhang, J.
Tissue Eng Part A 20: 1325-35 (2014), doi: 10.1089/ten.TEA.2013.0312.

Implantation of completely biological engineered grafts following decellularization into the sheep femoral artery
Syedain, Z.H.; Meier, L.A.; Lahti, M.T.; Johnson, S.L.; Hebbel, R.P; and Tranquillo, R. T.
Tissue Eng Part A 20(11-12): 1726-34 (2014), doi: 10.1089/ten.TEA.2013.0550.

Blood Outgrowth Endothelial Cells Alter Remodeling of Completely Biological Engineered Grafts Implanted into the Sheep Femoral Artery
Meier, L.A.; Syedain, Z.H.; Lahti, M.T.; Johnson, S.L.; Chen, M.H.; Hebbel, R.P.; and Tranquillo, R. T.
J Cardiovasc Trans Res 7(2): 242-9 (2014), doi: 10.1007/s12265-013-9539-z.

A multiscale approach to modeling the passive mechanical contribution of cells in tissues
Lai, V.K.; Hadi, M.F.; Tranquillo, R.T.; and Barocas, V.H.
J Biomech Eng 135(7): 71007 (2013), doi: 10.1115/1.4024350.

Combating adaptation to cyclic stretching by prolonging activation of extracellular signal-regulated kinase
Weinbaum, J.S.; Schmidt, J.B.; and Tranquillo, R.T.
Cell Molec Bioeng 6(3): 279-86 (2013), doi: 10.1007/s12195-013-0289-4.

Aligned human microvessels formed in 3D fibrin gel by constraint of gel contraction
Morin, K.T.; Smith, A.O.; Davis, G.E.; and Tranquillo, R.T.
Microvasc Res 90:12-22 (2013), doi: 10.1016/j.mvr.2013.07.010.

Tubular heart valves from decellularized engineered tissue
Syedain, Z.H.; Meier, L.A.; Reimer, J.; and Tranquillo, R.T.
Ann Biomed Eng 41(12): 2645-54 (2013), doi: 10.1007/s10439-013-0872-9.

Influence of cyclic mechanical stretch and tissue constraints on cellular and collagen alignment in fibroblast-derived cell sheets
Weidenhamer, N.K.; and Tranquillo, R. T.  
Tissue Eng Part C 19(5): 386-95 (2013), doi: 10.1089/ten.TEC.2012.0423.

Decellularized tissue-engineered heart valve leaflets with recellularization potential
Syedain, Z.H.; Bradee, A.R.; Kren S.; Taylor, D.A.; and Tranquillo,  R. T.
Tissue Eng Part A 19:759 (2013), doi: 10.1089/ten.TEA.2012.0365.  

Microstructural and mechanical differences between digested collagen-fibrin co-gels and pure collagen and fibrin gels
Lai, V. K.; Frey, C.R.; Kerandi, A.M.; Lake, S. P.; Tranquillo, R.T.; and Barocas, V.H.
Acta Biomat 8:4031 (2012), doi: 10.1016/j.actbio.2012.07.010.

Mechanical behavior of collagen-fibrin co-gels reflect transition from series to parallel interactions with increasing collagen content
Lai, V. K; Lake, S. P.; Frey, C.R.; Tranquillo, R.T.; and Barocas,  V.H.
J Biomech Eng 134: 011004-1 (2012), doi: 10.1115/1.4005544.

Hypoxic Culture and Insulin Yield Improvements to Fibrin-Based Engineered Tissue
Bjork, J.W.; Meier, L.A.; Johnson, S.L.; Syedain, Z.H.; and Tranquillo,  R.T.
Tissue Eng Part A, 18(7-8): 785-795 (2012)

Shear stress responses of adult blood outgrowth endothelial cells seeded on bioartifical tissue
Ahmann, K. A.; Johnson, S. L.; Hebbel, R.P.; and Tranquillo, R.T.
Tissue Eng Part A 17:2511 (2011); (cover photo),doi: 10.1089/ten.TEA.2011.0055.

Guided sprouting from endothelial spheroids in fibrin gels aligned by magnetic fields and cell-induced gel compaction
Morin, K.T.; and Tranquillo, R.T.
Biomaterials 32: 6111-6118 (2011), doi: 10.1016/j.biomaterials.2011.05.018.

Implantation of a Tissue-engineered Heart Valve from Human Fibroblasts Exhibiting Short Term Function in the Sheep Pulmonary Artery
Syedain, Z.H.; Lahti, M.T.; Johnson, S.L.; Robinson, P.S.; Ruth, G.R.; Bianco, R.W.; and Tranquillo, R.T.
Cardiovascular Engineering and Technology 2(2): 101-112 (2011), doi: 10.1007/s13239-011-0039-5

Ruthenium-catalyzed photo cross-linking of fibrin-based engineered tissue
Bjork, J.W.; Johnson, S.L.; and Tranquillo, R.T.
Biomaterials 32(10): 2479-2488 (2011), doi: 10.1016/j.biomaterials.2010.12.010.

TGF-B1 diminishes collagen production during long-term cyclic stretching of engineered connective tissue: Implication of decreased ERK signaling
Syedain, Z.H.; and Tranquillo, R.T.
JBiomech 44(5): 848-55 (2011), doi: 10.1016/j.jbiomech.2010.12.007

Initial fiber alignment pattern laters extracellular matrix synthesis in fibroblast populated fibrin gel cruciforms and correlates with predicted tension
Sander, E.A.; Barocas, V.H.; and Tranquillo, R.T.
Ann Biomed Eng  39(2): 714–729, (2010), doi: 10.1007/s10439-010-0192-2.

Implantable arterial grafts from human fibroblasts and fibrin using a multi-graft pulsed flow-stretch bioreactor with noninvasive strength monitoring
Syedain, Z.H.; Meier, L.A.; Bjork, J.W.; Lee, A.; and Tranquillo, R. T.
Biomaterials, 32(3): 714-22 (2011), doi: 10.1016/j.biomaterials.2010.09.019.

Fibrin Degradation Enhances Vascular Smooth Muscle Cell Proliferation and Matrix Deposition in Fibrin-Based Tissue Constructs Fabricated In Vitro
Ahmann, K.A., Weinbaum, J.S., Johnson, S.L., and R.T. Tranquillo
Tissue Eng Part A, 16(10): 3261-70 (2010). 

The Matrix-Binding Domain of Microfibril-associated Glycoprotein-1 Targets Active Connective Tissue Growth Factor to a Fibroblast-Produced Extracellular Matrix
Weinbaum, J.S., Tranquillo, R.T., and R.P. Mecham
Macromol Biosci, 10: 1338-44 (2010). 

Fusion of concentrically layered tubular tissue constructs increases burst strength
Huynh, T.N. and R.T. Tranquillo
Ann Biomed Eng, 38: 2226 (2010). 

Monitoring Collagen Transcription by Vascular Smooth Muscle Cells in Fibrin-Based Tissue Constructs
Weinbaum, J. S. and R. T. Tranquillo
Tissue Eng Part C , 16(3): 459-67 (2010). (abstract)

Controlled compaction with ruthenium-catalyzed photochemical cross-linking of fibrin-based engineered connective tissue
Syedain, Z.H., Bjork, J.W., Sando, L., and R.T. Tranquillo
Biomaterials, 30: 6695-701 (2009). 

Image-based multi-scale modeling predicts tissue-level and network-level fiber reorganization in stretched cell-compacted collagen gels
Sander, E.A., Stylianopoulos, T., Tranquillo, R. T., and V. H. Barocas
PNAS 106(42): 17675-80 (2009). 

Transmural Flow Bioreactor for Vascular Tissue Engineering
Bjork, J. W. and R. T. Tranquillo
Biotech & Bioeng, 104: 1197-206 (2009). 

A Bioreactor for Tissue Engineered Heart Valves Based on Controlled Cyclic Stretching 
Syedain, Z. and R. T. Tranquillo
Biomaterials, 30: 4078-84 (2009). 

Cell-induced alignment augments twitch force of fibrin gel-based engineered myocardium
Black, L.D., Meyers, J.D., Weinbaum, J.S., Shvelidze, Y.A. and R. T. Tranquillo
Tissue Eng Part A. 15: 3099-108 (2009). 

Planar Biaxial Behavior of Native and Tissue-Engineered Heart Valve Leaflets
Robinson, P.S. and R.T. Tranquillo
Tissue Eng Part A. 15: 2763-72 (2009). 

Planar Biaxial Mechanical Behavior of Bioartificial Tissues Possessing Prescribed Fiber Alignment
Jhun, C-S, Evas, M.C., Barocas, V.H. and R.T. Tranquillo
J Biomech Eng, 131: 081006-1-0810068 (2009 - cover photo).

Image-Based Biomechanics of Collagen Based Tissue Equivalents: Multiscale Models Compared To Fiber Alignment Predicted by Polarimetric Imaging
Sander, E.A., Stylianopoulos, T., Tranquillo, R.T., and V.H. Barocas
IEEE Eng Med Biol, 28(3): 10-18 (2009). 

Functional Tissue-Engineered Valves from Cell-Remodeled Fibrin with Commissural Alignment of Cell-Produced Collagen
Robinson, P. S., Johnson, S. L., Evans, M. C., Barocas, V.H. and R. T. Tranquillo
Tissue Eng Part A. 14: 83-95 (2008). 

Cyclic Distension of Fibrin-Based Tissue Constructs: Evidence of Adaptation During Growth of Engineered Connective Tissue
Syedain, Z., Weinberg, J.S., and R. T. Tranquillo,
PNAS 105:6537-6542 (2008). 

Cytokine Induction of Functional Smooth Muscle Cells from Multipotent Adult Progenitor Cells
Ross J.J., Zhigang H., Willenbring B., Zeng L., Isenberg B., Lee E.H., Reyes M., Keirstead S.A., Weir E.K., Tranquillo R.T., Verfaillie C.M.
J Clin Invest. 2006 116:3139-3149. 

Small-diameter artificial arteries engineered in vitro {Review}
Isenberg, B.C., Williams, C. and R.T. Tranquillo
Circ Res. 2006 Jan 6;98(1):25-35. 

Endothelialization and Flow Conditioning of Fibrin-based Media-equivalents
Isenberg, B.C., Williams, C. and R.T. Tranquillo
Ann Biomed Eng. 2006 Jun;34(6):971-85. 

Cell Sourcing for Fibrin-Based Valve Constructs
Williams, C., Johnson, S. L., Robinson, P. S. and R. T. Tranquillo
Tissue Eng. 2006 Jun;12(6):1489-502.

Tissue engineered valves with commissural alignment
Neidert, M.R. and Tranquillo, R.T.
Tissue Eng. 2006 Apr;12(4):891-903. 

Schwann cell behavior in three-dimensional collagen gels: Evidence for differential mechano-transduction and the influence of TGF-beta1 in morphological polarization and differentiation
Rosner, B. I., Hang, T-C., and R. T. Tranquillo
Exp Neurol. 2005 Sep;195(1):81-91.

Cryopreservation of collagen-based tissue equivalents. II. Improved freezing in the presence of cryoprotective agents.
Neidert MR, Devireddy RV, Tranquillo RT, Bischof JC.
Tissue Eng. 2004 Jan-Feb;10(1-2):23-32.

Rational design of contact guiding, neurotrophic matrices for peripheral nerve regeneration.
Rosner BI, Siegel RA, Grosberg A, Tranquillo RT.
Ann Biomed Eng. 2003 Dec;31(11):1383-401.

Cryopreservation of collagen-based tissue equivalents. I. Effect of freezing in the absence of cryoprotective agents.
Devireddy RV, Neidert MR, Bischof JC, Tranquillo RT.
Tissue Eng. 2003 Dec;9(6):1089-100.

Elastic fiber production in cardiovascular tissue-equivalents.
Long JL, Tranquillo RT.
Matrix Biol. 2003 Jun;22(4):339-50.

Long-term cyclic distention enhances the mechanical properties of collagen-based media-equivalents.
Isenberg BC, Tranquillo RT.
Ann Biomed Eng. 2003 Sep;31(8):937-49.

A fibrin-based arterial media equivalent. 
Grassl ED, Oegema TR, Tranquillo RT. 
J Biomed Mater Res. 2003 Sep 1;66A(3):550-61.

Temporal variations in cell migration and traction during fibroblast-mediated gel compaction.
Shreiber DI, Barocas VH, Tranquillo RT.
Biophys J. 2003 Jun;84(6):4102-14.

Fiber alignment imaging during mechanical testing of soft tissues.
Tower TT, Neidert MR, Tranquillo RT.
Ann Biomed Eng. 2002 Nov-Dec;30(10):1221-33.

Confined compression of a tissue-equivalent: collagen fibril and cell alignment in response to anisotropic strain.
Girton TS, Barocas VH, Tranquillo RT.
J Biomech Eng. 2002 Oct;124(5):568-75.

A novel implantable collagen gel assay for fibroblast traction and proliferation during wound healing.
Enever PA, Shreiber DI, Tranquillo RT. 
J Surg Res. 2002 Jun 15;105(2):160-72. 

Enhanced fibrin remodeling in vitro with TGF-beta1, insulin and plasmin for improved tissue-equivalents.
Neidert MR, Lee ES, Oegema TR, Tranquillo RT. 
Biomaterials. 2002 Sep;23(17):3717-31.

The tissue-engineered small-diameter artery. 
Tranquillo RT.
Ann N Y Acad Sci. 2002 Jun;961:251-4.

Macrophages influence a competition of contact guidance and chemotaxis for fibroblast alignment in a fibrin gel coculture assay.
Bromberek BA, Enever PA, Shreiber DI, Caldwell MD, Tranquillo RT. 
Exp Cell Res. 2002 May 1;275(2):230-42.

Fibrin as an alternative biopolymer to type-I collagen for the fabrication of a media equivalent.
Grassl ED, Oegema TR, Tranquillo RT.
J Biomed Mater Res. 2002 Jun 15;60(4):607-12.

Alignment maps of tissues: II. Fast harmonic analysis for imaging. 
Tower TT, Tranquillo RT. 
Biophys J. 2001 Nov;81(5):2964-71. 

Alignment maps of tissues: I. Microscopic elliptical polarimetry.
Tower TT, Tranquillo RT. 
Biophys J. 2001 Nov;81(5):2954-63. 

Neuronal contact guidance in magnetically aligned fibrin gels: effect of variation in gel mechano-structural properties.
Dubey N, Letourneau PC, Tranquillo RT. 
Biomaterials. 2001 May;22(10):1065-75. 

Effects of pdgf-bb on rat dermal fibroblast behavior in mechanically stressed and unstressed collagen and fibrin gels.
Shreiber DI, Enever PA, Tranquillo RT.
Exp Cell Res. 2001 May 15;266(1):155-66.

A self-consistent cell flux expression for simultaneous chemotaxis and contact guidance in tissues. 
Wagle MA, Tranquillo RT.
J Math Biol. 2000 Oct;41(4):315-30. 

Mechanisms of stiffening and strengthening in media-equivalents fabricated using glycation.
Girton TS, Oegema TR, Grassl ED, Isenberg BC, Tranquillo RT.
J Biomech Eng. 2000 Jun;122(3):216-23. 

Guided neurite elongation and schwann cell invasion into magnetically aligned collagen in simulated peripheral nerve regeneration.
Dubey N, Letourneau PC, Tranquillo RT.
Exp Neurol. 1999 Aug;158(2):338-50. 

Magnetically aligned collagen gel filling a collagen nerve guide improves peripheral nerve regeneration.
Ceballos D, Navarro X, Dubey N, Wendelschafer-Crabb G, Kennedy WR, Tranquillo RT.
Exp Neurol. 1999 Aug;158(2):290-300. 

Exploiting glycation to stiffen and strengthen tissue equivalents for tissue engineering. 
Girton TS, Oegema TR, Tranquillo RT.
J Biomed Mater Res. 1999 Jul;46(1):87-92. 

Self-organization of tissue-equivalents: the nature and role of contact guidance.
Tranquillo RT.
Biochem Soc Symp. 1999;65:27-42. 

A fibrin or collagen gel assay for tissue cell chemotaxis: assessment of fibroblast chemotaxis to GRGDSP.
Knapp DM, Helou EF, Tranquillo RT.
Exp Cell Res. 1999 Mar 15;247(2):543-53. 

Engineered alignment in media equivalents: magnetic prealignment and mandrel compaction.
Barocas VH, Girton TS, Tranquillo RT.
J Biomech Eng. 1998 Oct;120(5):660-6. 

A finite element solution for the anisotropic biphasic theory of tissue-equivalent mechanics: the effect of contact guidance on isometric cell traction measurement. 
Barocas VH, Tranquillo RT.
J Biomech Eng. 1997 Aug;119(3):261-8. 

An anisotropic biphasic theory of tissue-equivalent mechanics: the interplay among cell traction, fibrillar network deformation, fibril alignment, and cell contact guidance.
Barocas VH, Tranquillo RT.
J Biomech Eng. 1997 May;119(2):137-45.

Magnetically orientated tissue-equivalent tubes: application to a circumferentially orientated media-equivalent. 
Tranquillo RT, Girton TS, Bromberek BA, Triebes TG, Mooradian DL.
Biomaterials. 1996 Feb;17(3):349-57. 

Stochastic model of receptor-mediated cytomechanics and dynamic morphology of leukocytes.
Tranquillo RT, Alt W.
J Math Biol. 1996;34(4):361-412.

The fibroblast-populated collagen microsphere assay of cell traction force--Part 2: Measeurement of the cell traction parameter.
Barocas VH, Moon AG, Tranquillo RT.
J Biomech Eng. 1995 May;117(2):161-70